Perhaps most famous for her portrayal of Zareen on the Pakistani television series Main Abdul Qadir Hoon, Yousuf is also notable for her work in such Hum TV and and Geo TV series as Meray Dard Ko Jo Zuban Miley (2012) and Takkay ki Ayegi Baraat (2011).
At the start of her entertainment career, she worked as a VJ for Pakistan's AAG TV channel and also acted in several television commercials.
In 2011, she competed on the reality television show Desi Kuriyan.
Raised in Karachi, Pakistan, as a member of a Muslim family, she is the sister of actress and model Syra Yousuf and VJ Palwasha Yousuf. Following their 2009 wedding, Alishba Yousuf and her husband Rayyan Durrani welcomed a daughter named Iliyana.
She and Javed Sheikh were both in the cast of the 2012 Pakistani drama series Annie Ki Ayegi Baraat.