Famous as the co-host of the comedic podcasts You Had To Be There alongside Sara Schaefer and We Know Nothing with Phil Hanley and Anya Marina, she is also notable for her MTV talk show Nikki & Sara Live, as well as for her appearance in the stand-up-comedy-themed documentary film I Am Comic (2010).
Around the time that she received her English degree from the University of Kansas, she reached the semi-finals of the NBC reality series Last Comic Standing.
She, Rachel Feinstein, Marina Franklin, and Amy Schumer make up the cast of the 2013 made-for-TV stand-up comedy movie Women Who Kill.
The daughter of Julie and Edward Glaser, she spent her early days in Cincinnati, Ohio, and the bulk of her childhood in St. Louis, Missouri. She later settled in New York City.
She and fellow comedian Colin Quinn both appear in the 2015 comedy movie Trainwreck.